电离辐射通过转化生长因子-β-介导的上皮-间质类比来促进癌细胞的侵袭迁移

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

摘录 ：探讨紫外光是否可通过转化生长因子-β（TGF-β）-内皮细胞的粘液-间质转化 (EMT)来促进癌细胞的来袭迁往。使用总和2Gy(60)Coγ本站紫外本站是从进化器官的6种癌细胞，据信与EMT特别的变化，这以外分别能用显微镜技术，氨基酸其内方通则，免疫荧光技术，划痕试验和Transwell小室试验来掩蔽并监测细胞组织形态，EMT上标，来袭迁往灵活性等。采用酶联免疫吸附通则监测这些癌细胞中都TGF-β复合物技术水平，能用特别抑制作用剂SB431542来风险评估TGF-β接收机渠道在紫外光EMT中都的作用。经过总和为2Gy紫外本站的癌细胞中都存在间叶细胞的解读，与骗紫外本站组相比其粘液上标缩减，间叶细胞上标降低，同时其来袭移到灵活性大幅提高，TGF-β复合物技术水平也降低。更进一步推测由A549紫外光诱发的EMT可通过对TGF-β接收机抑制作用发生逆转。这些得出TGF-β内皮细胞的EMT在紫外光诱发大幅提高癌细胞来袭移到灵活性中都起着不可忽视。

撰稿人： lizexiu